Development of basil ‘Dark Opal’ from explant type and plant regulators

Seedling abnormalities are a common problem in micropropagation and require investigation. This study aimed to assess the growth characteristics morphoanatomy of stem apices nodal segments basil (Ocimum basilicum) ‘Dark Opal’ inoculated medium containing combinations 6-benzylaminopurine (BAP) naphthaleneacetic acid (NAA). Presence calluses, adventitious roots, abnormal seedlings was quantified at 20 40 days after inoculation. Growth, anatomical, morphological parameters were evaluated 80 days. Explant type regulators influenced Opal’. Treatment with 0.05 0.1 mg L-1 NAA BAP increased leaf number, fresh weight, dry weight decreased incidence calluses abnormalities. Hyperhydric showed morphoanatomical alterations, such as disorganization expansion intercellular spaces consequent increase parenchymal length. Thus, results this research allowed optimizing understanding important effects on development species, which will help vitro mass culture programs biofactories for production secondary metabolites.